Limited proteolysis of the porcine zona pellucida by homologous sperm acrosin
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چکیده
منابع مشابه
Caprine acrosin. Purification, characterization and proteolysis of the porcine zona pellucida.
Acrosin purified from an acidic extract of ejaculated goat spermatozoa migrated as a single 42,000-Mr band in SDS/polyacrylamide-gel electrophoresis. Reduction and alkylation of caprine acrosin produced two polypeptides, one of Mr 40,000 (heavy chain) and the other of Mr 3700 (light chain). The light chain purified by reversed-phase h.p.l.c. was a glycosylated octadecapeptide with an amino acid...
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Experiments were designed to characterize the effect of progesterone and the zona pellucida (ZP) on the goat sperm acrosome reaction (AR) through a comparative study. Goat spermatozoa were incubated for 4h in Krebs-Ringer bicarbonate media (KRB) for capacitation. Progesterone and ZP stimulated exocytosis of capacitated spermatozoa in a dose-dependent manner. EGTA and La(3+), added 10min before ...
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The zona pellucida (ZP) in a majority of species consists of three antigenic families with wide inter species cross reactivity. The glocoproteins of heat solubilized goat zona pellucida (gZP) were isolated into three families by high performance liquid chromatography on a gel ®ltration column. Their molecular identities were 180, 95 and 75 kDa for gZP1, gZP2 and gZP3 respectively. However under...
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branes, was measured by the method of Abrams [S]. Endopeptidase activity was measured by incubating 50 pl of each fraction with 450 pI of peptide-coumarin derivative, each at 0.1 11 mM in 50 mM-Tris-HCI, pH 7.5. The release of free 7-amino-4-methylcoumarin was quantified fluorimetrically using excitation and emission wavelengths of 370 nm and 440 nm, respectively. The results obtained for S. cr...
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For mammalian spermatozoa to exhibit the ability to bind the zona pellucida (ZP) they must undergo three distinct phases of maturation, namely, spermatogenesis (testis), epididymal maturation (epididymis) and capacitation (female reproductive tract). An impressive array of spermatozoa surface remodeling events accompany these phases of maturation and appear critical for recognition and adhesion...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1985
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0740257